Details of Mendel's Pea Breeding
Here is a copy of
Mendel's
original paper, for those who are interested.

The following pea
breeding illustrations were obtained from the Eighth Edition of Biology
by Neil A. Campbell. I've scanned the relevant illustrations about pea
breeding. If you would like to download the genetics
section in PDF form instead, then here you go:
part1-- part2 -- part3 -- part4
You can also read the whole genetics chapter in a virtual pdf online
with virtual page turning instead here.
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Rebsie Fairholm's Red-Podded Pea

If your
still interested in pea breeding, then you might be interested in
Rebsie Fairholm's breeding projects involving peas. Not only is she
breeding a very neat yellow sugar snap pea called Luna
Trick, but she is also breeding an awesome red-podded
pea
as well! She not only shares photos and info about her crosses on her
blog, but she has also provided 2 excellent tutorials for crossing peas
with photos!
Trying to figure out
the gentics for
this rare red-podded pea is facinationg! Here are my attemopts to
figure it all
out with punnett squares below.
Parent Generation (P)
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But as it turns out,
Rebsie's results actually had mostly green pods. And upon doing some
research about the genes responsible for the purple-podded trait, we
actually find that there may instead be 3 genes needed
for the anthocyanins to be present. One gene commonly called "A" is a
master swich gene and is epistatic to the other genes coding for
anthocyanins. The other two genes are also both required for the pod to
have purple-pod's. If this is correct than that means the punnit
squares i completed above are no where close to being accurate. Here is
the F1 hypothesis again, and this one as far as i know is correct this
time. I have used the letter "A" to represent the on/off gene, along
with "P" and "U" to represent the two purple-pod genes. I have left out
the yellow podded gene because all offspring will be hetozygous for a
base pod color of yellow/green.
Here is the corrected F1 generation hypothesis using the three genes
for purple anthocyanin colors. We are ignoring the gene for
green/yellow pods for the moment since all offspring in the F1
generation are heterozygous for dominant green and recessive yellow.
F1
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APU
1/8
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aPU
1/8
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ApU
1/8
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APu
1/8
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apU
1/8
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aPu
1/8
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Apu
1/8
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apu
1/8
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APU
1/8
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AAPPUU
Purple!
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AaPPUU
Purple! |
AAPpUU
Purple! |
AAPPUu
Purple! |
AaPpUU
Purple! |
AaPPUu
Purple!
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AAPpUu
Purple!
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AaPpUu
Purple!
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aPU
1/8
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AaPPUU
Purple! |
aaPPUU
Green
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AaPpUU
Purple! |
AaPPUu
Purple! |
aaPpUU
Green
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aaPPUu
Green
|
AaPpUu
Purple!
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aaPpUu
Green
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ApU
1/8
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AAPpUU
Purple! |
AaPpUU
Purple! |
AAppUU
Purple!
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AAPpUu
Purple! |
AappUU
Green
|
AaPpUu
Purple!
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AAppUu
Green
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AappUu
Green
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APu
1/8
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AAPPUu
Purple! |
AaPPUu
Purple! |
AAPpUu
Purple!
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AAPPuu
Green
|
AaPpUu
Purple! |
AaPPuu
Green
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AAPpuu
Green
|
AaPpuu
Green
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apU
1/8
|
AaPpUU
Purple! |
aaPpUU
Green
|
AappUU
Green
|
AaPpUu
Purple! |
aappUU
Green
|
aaPpUu
Green
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AappUu
Green
|
aappUu
Green
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aPu
1/8
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AaPPUu
Purple! |
aaPPUu
Green
|
AaPpUu
Purple! |
AaPPuu
Green
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aaPpUu
Green
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aaPPuu
Green
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AaPpuu
Green
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aaPpuu
Green
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Apu
1/8
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AAPpUu
Purple! |
AaPpUu
Purple! |
AAppUu
Green
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AAPpuu
Green
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AappUu
Green
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AaPpuu
Green
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AAppuu
Green
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Aappuu
Green
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apu
1/8
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AaPpUu
Purple! |
aaPpUu
Green
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AappUu
Green
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AaPpuu
Green
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aappUu
Green
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aaPpuu
Green
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Aappuu
Green
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aappuu
Green
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Here is the F2 Generation Hypothesis using the rule of independent
assortment. Now
this table is not entirely correct, but represents the "average"
offspring collected from the purple-podded plants in the F1 generation.
I say the average because in the best case scenario you can get
purple-podded plants that be homozygous for ALL of the purple
genes. On the other hand, the worst case scenario is that the
purple-podded plants in the F1 will be heterozygous for ALL of the
purple genes. In most cases though i think that the average
purple-podded plant in the F1 will have two homozygous genes and the
third gene will be heterozygous. In that case you would only need to
worry about two sets of genes in the F2, nameley 1 set for anthocyanin
colors and 1 set for yellow pods underneath.
F2
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GP
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Gp
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yP
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yp
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GP
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GGPP

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GGPp

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GyPP

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GyPp

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Gp
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GGPp

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GGpp

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GyPp

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Gypp

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yP
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GyPP

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GyPp

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yyPP

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yyPp

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yp
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GyPp

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Gypp

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yyPp

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yypp

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Download F2 table
here
Which in this "average" scenario results in the typical 9:3:3:1
Phenotypic Ratio.
And in this case the red-podded peas are the recombinant offspring that
we are loking for.
If we take that a little furthur, that means that if you plant 50 F2 generation seeds,
you should get a ratio of about 43 non-red pods : 7 red-pods.
Inheritance Of The Colors Of Pea Flowers
Pea flowers (the
edible kind) come in three major colors. They can come in the "wild"
form which is a Bicolour Purple, White, or Salmon Pink
(pink-and-white). I first encountered this information on
Rebsie's blog, and after doing some research of my own, i found one refrence to the same imformation in a very old book from 1912 (
Breeding and the Mendelian discovery by A.D. Darbishire).
The purple form is dominant and is a trait mostly common in field peas.
The pink form is recessive to the the purple, but is dominant to the
white. The white form is recessive to all color, and is commonly
associated with modern peas that have been selected for high sugar
content. It's a bit amusing the way the book talks about the purple
form in relation to the other two. Apparently if you breed the pink
with the white you will get purple in the F1 generation because the
pink has the gene that expresses color, but the white is actually
hiding the gene for purple flowers. In the book this is talked about as
an ancestral trait, a throwback, and the theory of reversion.
In
Darwins book,
The Origin of Species,
Darwin himself encounters something similar with his breeding of
pigeons. Darwin bred a pure white pigeon with another white pigeon
(with black tail feathers), and was very surprised because in the next
generation he got a blue pigeon (which has the same coloring as the
wild rock pigeon). But Darwin didn't know about genetics, so he could
only conclude that it was a ancestral throwback phenomenon. We now know
that the white one with black stripes had the gene for color (black)
and the pure white pidgeon was actually a blue pidgeon but did not have
any active color genes. To my knowlwdge the only variety of pea known to have pink flowers is the one called 'Salmon-flowered'.