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Pea Breeding


Pea Breeding is actually really awesome. Especially when you can get really neat colors to recombine into new combinations. Punnett Squares to predict the genetics of pea breeding is also very helpful and fun too. This page contains a multitude of information on pea gentics. For brief info about the gentics about the rare pea traits I'm currently studying, then please visit my original pea page.


Details of Mendel's Pea Breeding

Here is a copy of Mendel's original paper, for those who are interested.



The following pea breeding illustrations were obtained from the Eighth Edition of Biology by Neil A. Campbell. I've scanned the relevant illustrations about pea breeding. If you would like to download the genetics section in PDF form instead, then here you go:

part1-- part2 -- part3 -- part4

You can also read the whole genetics chapter in a virtual pdf online with virtual page turning instead here.

crossing pea plants


F1 hybrid pea plants

The Results of Mendels F1 Crosses for Seven Characters in Pea Plants

peas, F2 generation
Random combination of the gametes results in the 3:1 ration that Mendel observed in the F2 generation.


Mendel Peas Testcross


Mendel independent assortment


Rebsie Fairholm's Red-Podded Pea



If your still interested in pea breeding, then you might be interested in Rebsie Fairholm's breeding projects involving peas. Not only is she breeding a very neat yellow sugar snap pea called Luna Trick, but she is also breeding an awesome red-podded pea as well! She not only shares photos and info about her crosses on her blog, but she has also provided 2 excellent tutorials for crossing peas with photos!

How to breed your own garden peas
How to hybridise garden peas 


Trying to figure out the gentics for this rare red-podded pea is facinationg! Here are my attemopts to figure it all out with punnett squares below.

Parent Generation (P)



F1 generation if purple-pod parent is homozygous for the purple gene
F1
yp
yp



GP

GyPp
purple pea
GyPp
purple pea



GP

GyPp
purple pea
GyPp
purple pea









OR
F1 generation if purple-pod parent is heterozygous for the purple gene
F1 yp
yp



GP

GyPp
purple pea
GyPp
purple pea



Gp

Gypp
green pea
Gypp
green pea




    But as it turns out, Rebsie's results actually had mostly green pods. And upon doing some research about the genes responsible for the purple-podded trait, we actually find that there may instead be 3 genes needed for the anthocyanins to be present. One gene commonly called "A" is a master swich gene and is epistatic to the other genes coding for anthocyanins. The other two genes are also both required for the pod to have purple-pod's. If this is correct than that means the punnit squares i completed above are no where close to being accurate. Here is the F1 hypothesis again, and this one as far as i know is correct this time. I have used the letter "A" to represent the on/off gene, along with "P" and "U" to represent the two purple-pod genes. I have left out the yellow podded gene because all offspring will be hetozygous for a base pod color of yellow/green.

Here is the corrected F1 generation hypothesis using the three genes for purple anthocyanin colors. We are ignoring the gene for green/yellow pods for the moment since all offspring in the F1 generation are heterozygous for dominant green and recessive yellow.

F1
APU
1/8
aPU
1/8
ApU
1/8
APu
1/8
apU
1/8
aPu
1/8
Apu
1/8
apu
1/8
APU
1/8
AAPPUU
Purple!
AaPPUU
Purple!
AAPpUU
Purple!
AAPPUu
Purple!
AaPpUU
Purple!
AaPPUu
Purple!
AAPpUu
Purple!
AaPpUu
Purple!
aPU
1/8
AaPPUU
Purple!
aaPPUU
Green
AaPpUU
Purple!
AaPPUu
Purple!
aaPpUU
Green
aaPPUu
Green
AaPpUu
Purple!
aaPpUu
Green
ApU
1/8
AAPpUU
Purple!
AaPpUU
Purple!
AAppUU
Purple!
AAPpUu
Purple!
AappUU
Green
AaPpUu
Purple!
AAppUu
Green
AappUu
Green
APu
1/8
AAPPUu
Purple!
AaPPUu
Purple!
AAPpUu
Purple!
AAPPuu
Green
AaPpUu
Purple!
AaPPuu
Green
AAPpuu
Green
AaPpuu
Green
apU
1/8
AaPpUU
Purple!
aaPpUU
Green
AappUU
Green
AaPpUu
Purple!
aappUU
Green
aaPpUu
Green
AappUu
Green
aappUu
Green
aPu
1/8
AaPPUu
Purple!
aaPPUu
Green
AaPpUu
Purple!
AaPPuu
Green
aaPpUu
Green
aaPPuu
Green
AaPpuu
Green
aaPpuu
Green
Apu
1/8
AAPpUu
Purple!
AaPpUu
Purple!
AAppUu
Green
AAPpuu
Green
AappUu
Green
AaPpuu
Green
AAppuu
Green
Aappuu
Green
apu
1/8
AaPpUu
Purple!
aaPpUu
Green
AappUu
Green
AaPpuu
Green
aappUu
Green
aaPpuu
Green
Aappuu
Green
aappuu
Green

That gives us a ratio of 28 Purple : 36 Green.
So I guess Rebsie was right; in the F1 generation mostly green pods appear.

    Here is the F2 Generation Hypothesis using the rule of independent assortment. Now this table is not entirely correct, but represents the "average" offspring collected from the purple-podded plants in the F1 generation. I say the average because in the best case scenario you can get purple-podded plants that be homozygous for ALL of the purple genes. On the other hand, the worst case scenario is that the purple-podded plants in the F1 will be heterozygous for ALL of the purple genes. In most cases though i think that the average purple-podded plant in the F1 will have two homozygous genes and the third gene will be heterozygous. In that case you would only need to worry about two sets of genes in the F2, nameley 1 set for anthocyanin colors and 1 set for yellow pods underneath.

F2
GP
Gp
yP
yp



GP
GGPP
purple pea
GGPp
purple pea
GyPP
purple pea
GyPp
purple pea



Gp
GGPp
purple pea
GGpp
green pea
GyPp
purple pea
Gypp
green pea



yP
GyPP
purple pea
GyPp
purple pea
yyPP
red pea
yyPp
red pea



yp
GyPp
purple pea
Gypp
green pea
yyPp
red pea
yypp
yellow pea

Download F2 table here

Which in this "average" scenario results in the typical 9:3:3:1 Phenotypic Ratio.
And in this case the red-podded peas are the recombinant offspring that we are loking for.
If we take that a little furthur, that means that if you plant 50 F2 generation seeds,
you should get a ratio of about 43 non-red pods : 7 red-pods.


Inheritance Of The Colors Of Pea Flowers

Mendelian Inheritance Of The Colour OF The Flower In The Culinary Pea

Pea flowers (the edible kind) come in three major colors. They can come in the "wild" form which is a Bicolour Purple, White, or Salmon Pink (pink-and-white). I first encountered this information on Rebsie's blog, and after doing some research of my own, i found one refrence to the same imformation in a very old book from 1912 (Breeding and the Mendelian discovery by A.D. Darbishire). The purple form is dominant and is a trait mostly common in field peas. The pink form is recessive to the the purple, but is dominant to the white. The white form is recessive to all color, and is commonly associated with modern peas that have been selected for high sugar content. It's a bit amusing the way the book talks about the purple form in relation to the other two. Apparently if you breed the pink with the white you will get purple in the F1 generation because the pink has the gene that expresses color, but the white is actually hiding the gene for purple flowers. In the book this is talked about as an ancestral trait, a throwback, and the theory of reversion.

In Darwins book, The Origin of Species, Darwin himself encounters something similar with his breeding of pigeons. Darwin bred a pure white pigeon with another white pigeon (with black tail feathers), and was very surprised because in the next generation he got a blue pigeon (which has the same coloring as the wild rock pigeon). But Darwin didn't know about genetics, so he could only conclude that it was a ancestral throwback phenomenon. We now know that the white one with black stripes had the gene for color (black) and the pure white pidgeon was actually a blue pidgeon but did not have any active color genes. To my knowlwdge the only variety of pea known to have pink flowers is the one called 'Salmon-flowered'.

Salmon-flowered, pink, pink-and-white pea flower
Bicolour Purple pea flower
white pea flower


peas pea collection